Table 1 The primer sequences for qRT-PCR.
Genes | Primers | |
|---|---|---|
Homo sapiens | β-actin | Forward: 5’-GGACTTCGAGCAAGAGATGG-3’ |
Reverse: 5’ -AGCACTGTGTTGGCGTACAG-3’ | ||
PPARD | Forward:5’- GCACTTCCTTCCAGCAGCTA-3’ | |
Reverse: 5’- TGAACACCGTAGTGGAAGCC-3’ | ||
PPARg | Forward: 5’- GCCCAGGTTTGCTGAATGTG-3’ | |
Reverse: 5’-TTGGCAAACAGCTGTGAGGA − 3’ | ||
PPARA | Forward: 5’-GCGAACGATTCGACTCAAGC − 3’ | |
Reverse: 5’-CATCCCGACAGAAAGGCACT − 3’ | ||
ACACA | Forward: 5’-CCCAACCTCTGGAGCTGAAC − 3’ | |
Reverse: 5’-CAAGGCCAAGCCATCCTGTA − 3’ | ||
ACOX1 | Forward: 5’- CGCCGAGAGATCGAGAACAT-3’ | |
Reverse: 5’- GCACTTTTCCTGACAGCCAC-3’ | ||
AMPK | Forward: 5’- ATGCGCAGACTCAGTTCCTG-3’ | |
Reverse: 5’- CTTCACTTTGCCGAAGGTGC-3’ | ||
ADIPOQ | Forward: 5’- GAGAACCTGGAGAAGGTGCC-3’ | |
Reverse: 5’-CCCAGGAATGTTGCAGTGGA − 3’ | ||
Mus musculus | AMPK | Forward: 5’-CAAGGACACGGGCATCTCTT-3’ |
Reverse: 5’-GGCTTGTAGAGGAGGGTGGT-3’ | ||
CPT1 | Forward: 5’-CTGAGCCATGAAGCCCTCAA-3’ | |
Reverse: 5’-ACGCCACTCACGATGTTCTT-3’ | ||
IL17A | Forward: 5’- TACCTCAACCGTTCCACGTC-3’ | |
Reverse: 5’-TTTCCCTCCGCATTGACACA − 3’ | ||
IL6 | Forward:5’-CCCCAATTTCCAATGCTCTCC − 3’ | |
Reverse: 5’-AGGTTTGCCGAGTAGATCTCAA − 3’ | ||
IL23 | Forward: 5’- CCTCCATGTGCACTTCCTGT-3’ | |
Reverse: 5’-CGGATCTGTCTGTAGCGGTC − 3’ | ||
TNF-α | Forward: 5’- CCCTCACACTCACAAACCAC-3’ | |
Reverse: 5’-ACAAGGTACAACCCATCGGC − 3’ | ||
ADIPOQ | Forward: 5’-AGCCGCTTATGTGTATCGCT − 3’ | |
Reverse: 5’-GAGTCCCGGAATGTTGCAGT − 3’ | ||
Gapdh | Forward: 5’- CATCACTGCCACCCAGAAGACTG-3’ | |
Reverse: 5’-ATGCCAGTGAGCTTCCCGTTCAG − 3’ |
