Table 2 Quantitative real-time PCR (RT-qPCR) primer sequences.
Gene | Sequences (5’-3’) | Amplicon size (bp) | Annealing (°C) | Efficiency% | Accession no. |
|---|---|---|---|---|---|
β-actin | F: AGCAAGCAGGAGTACGATGAG | 135 | 59 | 98 | XM_003443127.5 |
R: TGTGTGGTGTGTGGTTGTTTTG | |||||
IGF-1 | F: GTGGACGAGTGCTGCTTC | 139 | 58 | 98 | XM_019346352.2 |
R: TGCTACTAACCTTGGGTGC | |||||
SOD | F: GACGTGACAACACAGGTTGC | 198 | 60 | 99 | XM_003449940.5 |
R: TACAGCCACCGTAACAGCAG | |||||
CAT | F: TCCTGAATGAGGAGGAGCGA | 189 | 60 | 96 | JF801726 |
R: AAACGTGCAAAGTGGCATCC | |||||
GSH-Px | F: CCAAGAGAACTGCAAGAACGA | 237 | 58 | 97 | NM_001279711.1 |
R: CAGGACACGTCATTCCTACAC | |||||
IL-1β | F: TGCTGAGCACAGAATTCCAG | 172 | 60 | 99 | XM_019365841.2 |
R: GCTGTGGAGAAGAACCAAGC | |||||
TNF-1α | F: CCAGAAGCACTAAAGGCGAAGA | 82 | 60 | 97 | AY428948.1 |
R: CCTTGGCTTTGCTGCTGATC |
