Fig. 6
From: Maltose metabolism in serum free CHO culture involves lysosomal acid α-glucosidase

Overexpression of GAA in GAA-KO CHO-K1: growth and metabolite profiles of stably transfected GAA-KO CHO-K1 cells. CHO-K1 cells originally cultivated in glucose-containing OptiCHO were subsequently grown in OptiCHO with 10 g/l of maltose. The cultures were monitored at 0, 48 and 96 h post-inoculation to obtain their (a) viable cell densities (────) and cell viabilities (┅┅┅┅) and (b) relative intracellular maltose concentrations at 48 and 96 h. Mean and standard deviations values from 2 independent replicates are plotted.