Table 3 Effect of humanin supplementation on sperm plasma membrane integrity at fresh, pre-freeze and post-thaw of cryopreservation (Mean ± SE; n = 24).

From: Mitochondrial derived peptide humanin improved semen quality, semen freezability, antioxidant status and in-vitro fertility in crossbred bull

Stages

Plasma membrane integrity

Group I

(control)

Group II

(2.5 µM)

Group III

(5 µM)

Group IV

(7.5 µM)

Fresh

83.85 ± 1.78A

83.85 ± 1.78A

83.85 ± 1.78A

83.85 ± 1.78A

Pre-freeze

71.50 ± 1.72Bb

73.79 ± 1.80Bb

79.42 ± 1.40Aa

78.92 ± 1.30Aa

Post-thaw

49.31 ± 1.46Cc

50.95 ± 1.28Cbc

60.76 ± 1.73Ba

58.25 ± 2.05Bab

Decrease (%) from

fresh to pre-freeze stage

14.73

12

5.28

5.9

Decrease (%) from

fresh to post-thaw stage

41.19

39.67

27.54

30.53

  1. Means bearing different superscripts in lower case (a, b and c) in a row differ significantly (p < 0.05). Means bearing different superscripts in upper case (A, B and C) in a column differ significantly (p < 0.05). Number of ejaculates = 24.
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