Table 1 Primers used for genetic stability assessment.
S. no. | Primer name | Sequence (3’-5’) | Annealing Tm | Number of amplified bands | Band size (bp) |
|---|---|---|---|---|---|
1. | IS6 | (GA)8C | 51.2 °C | 3 | 100–900 |
2. | IS7 | (GT)8A | 50.7° | 2 | 200, 400 |
3. | IS8 | (AG)8C | 51.2° | 5 | 100–700 |
4. | IS9 | (TG)7TA | 53.6° | 3 | 200–500 |
5. | IS11 | (CA)7G | 52.2° | 1 | 300 |
6. | IS12 | (GT)8C | 52.2° | 1 | 200 |
7. | IS53 | (AG)7C | 54.0° | 3 | 200–600 |
8. | IS61 | (GA)8T | 54.0° | 3 | 200–900 |
9. | IS65 | (AG)8T | 52.8° | 2 | 200, 500 |
10. | UBC814 | (CT)8A | 50° | - | – |
11. | UBC819 | (GT)8A | 50° | - | – |
12. | UBC820 | (GT)8C | 50° | - | – |
13. | UBC825 | (AC)8T | 52.8° | 2 | 100, 500 |
14. | UBC826 | (AC)8C | 52.8° | 2 | 200, 500 |
15. | UBC828 | (TG)8A | 50° | 2 | 100, 400 |
16. | UBC834 | (AG)8CT | 50.6° | - | – |
17. | UBC835 | (AG)8TC | 50.6° | - | – |
18. | UBC836 | (AG)8TA | 50° | - | – |
19. | UBC840 | (GA)8CT | 50° | 1 | 200 |
20. | UBC841 | (GA)8TC | 53.6° | 2 | 100, 400 |
21. | UBC842 | (GA)8TG | 53.6° | 2 | 200, 600 |
22. | UBC847 | (CA)8AC | 50° | 3 | 100–500 |
23. | UBC848 | (CA)8AG | 50° | 2 | 100, 500 |
24. | UBC853 | (TC)8AT | 50° | 3 | 100–600 |
25. | UBC856 | (AC)8CA | 50° | 2 | 300, 700 |
26. | UBC858 | (TG)8AT | 50.8° | 1 | 300 |
27. | UBC859 | (TG)8AC | 52.2° | 2 | 300, 500 |
28. | UBC870 | (TGC)6 | 51.2° | 2 | 100, 500 |
29. | UBC871 | T(ATT)6 | 51.2° | 3 | 100–600 |
30. | UBC872 | (GATA)4 | 51.2° | 3 | 200–700 |
