Fig. 4
Microviscosity of normal mouse breast tissues and human breast cancer assessed from FLIM with BODIPY2. (A) Representative histological, fluorescence intensities and FLIM images of normal and cancer tissue samples stained with the BODIPY2 fluorescent molecular rotor. Scale bar: 60 μm. (B) Quantification of microviscosity in the tissues. Mean ± SD, n = 50 cells. Dot scatter plot displaying measurements for individual cell membranes (dots) and the mean (horizontal lines) of microviscosity in different samples. Importantly, within the tissue only the regions of interest where the rotor demonstrated a monoexponential fluorescence decay (χ2 ≤ 1.40) were analyzed, which indicated its correct operation. (C) Representative fluorescence decay curves of BODIPY2 in normal breast duct cells and MCF-7 tumor cells.
