Fig. 3
BMV proteins co-expression network identified age-dependent modules which are important for BBB function. (A) The correlation between modules and age. Heatmaps show the correlation between eigengene and age and each cell contains the corresponding correlation followed by p-value. (B) Module eigenprotein levels by age groups (Development, Adult, Elderly) for the three blood brain barrier related modules. Modules are grouped by different age groups. (C) Macronutrient transporters and transporters involving in macronutrient metabolism in our BMV proteomic dataset are shown in bar graphs. Amino acid transporters are labeled as black, sugar transporters are labeled as green and choline transporters are labeled as orange. (D) Micronutrient transporters in our BMV proteomic dataset are shown in a circular bar graph, bars represent the mean of the transporter expressions in different age groups. For the bar graphs, Kruskal–Wallis tests followed by Dunn’s post hoc test were used to compare the mean of each age groups with the mean of the Adult group. Data are represented as mean ± SEM and each point represents one sample. *P < 0.05, **P < 0.01, ***P < 0.001. ND, not detected in more than 30% of samples in specific age group.
