Fig. 5 | Scientific Reports

Fig. 5

From: Pharmacological activation of SERCA2 reverses ER calcium dysregulation and depression-like behaviors in hyperglycemic mice

Fig. 5

Intrahippocampal inhibition of SERCA2 by thapsigargin induces depression-like behaviors. (A) Timeline of experimental procedures. (B) Microinfusions targeted to the dentate gyrus (DG) of the hippocampus. A total of 0.5 µl of 20 µM TG was injected bilaterally per side. (C) Female urine and (D) water sniffing time in the FUST. (E) Immobile time in the TST. (F) Time spent in the center zone and (G) total locomotor activity in the OFT. (H) SERCA2 expression in the DG. (I) Phosphorylated IRE1α levels. (J) CHOP and cleaved caspase-12 levels. (K) Bax and cleaved caspase-3 levels. All analyses were performed in the DG. Protein levels were quantified relative to β-actin or corresponding total forms, and levels are shown as fold changes relative to those of the control group. Original blots are presented in Supplementary Information 2 (H-K). *p < 0.05, **p < 0.01, ***p < 0.001. All data are mean ± SEM. Detailed statistics are in Supplementary Information 1, Table S3.

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