Fig. 3
From: Characterization of a cis-regulatory element upstream of matrix metalloproteinase-9
SDDs across E1 identify segments that change luciferase reporter activity. (a) HD1-HD11 deletion constructs were designed in the human E1-pGL4.23 based on the pattern of predicted TF binding sites in the JASPAR track of the UCSC genome browser40,41,44 (Supplementary Fig. S4). (b) HD1, HD9, and HD10 (blue bars) have significantly decreased luciferase reporter activity compared to E1, and HD11 (orange bar) has significantly increased luciferase reporter activity compared to E1. The x-axis shows luciferase activity relative to the empty pGL4.23 vector. The black dots in (b) are individual biological replicate values for the experiment. (**) indicates p-value < 0.01, and (***) indicates p-value < 0.001. All experiments were performed with three biological replicates. Results are displayed as the mean +/- standard error (SE), and p-values were calculated using a two-tailed student’s t-test.
