Table 5 Nucleotide sequences of primers used for qRT-PCR analysis of H. pylori virulence genes.
Gene | Primer sequence (5′→3′) | Product size (bp) | Anealing (°C) |
|---|---|---|---|
cagA | F: AGCAAAAAGCGACCTTGAAA R: AGCCAATTGCTCCTTTGAGA | 172 | 60 |
vacA | F: CGCCTTCTTTACAACCGTGAT R: AACCTCTTCCTGCTTGAATGC | 170 | 60 |
ureA | F: GAAGACATCACTATCAACGAAGGCAA R: GTTACCGCCAATGTCAATCAA | 231 | 60 |
spoT | F: GCGTGTCTTCCACCACATCAT R: GCGAGCCTTATATTGTCCATCCT | 111 | 60 |
hspR | F: ACCCTCAAACCTTGCGCCAATATG R: TCCACGCCCGCTAGATTAACCC | 154 | 60 |
16S rRNA | F: GCTCTTTACGCCCAGTGATTC R: GCGTGGAGGATGAAGGTTTT | 152 | 60 |
