Fig. 5
eIF4E pathway, a critical factor for chemoresistance. MDAN and MDAR cells were stimulated with dox increasing concentrations (0–1.6.6 µM) at 48 h treatment; (A) Western blot analysis of the scaffold protein eIF4G and helicase protein eIF4A and their densitometric analysis (B) and (C) respectively. (D)Western-blot of VEGF expression in both variants, GAPDH was used as a loading control. (E) Densitometry analysis of VEGF. (F) Metalloproteinase-9 activity evaluated by zymography and respective densitometric analysis (G); results showed the mean and standard deviation (X ± S.D.) of three biological replicates. To assess this process, MDAN control sample was used as control. (H) Representative images of cell invasion experiments in MDAN and MDAR cells under treatment with increasing dox concentrations (0–1.6.6 µM) and concomitant FBS (10%). (I) Densitometric analysis of invasion assays in the MDAN and MDAR variant. Results showed three biological replicates’ mean and standard deviation (n = 3, X ± S.D.). To evaluate this process, DMEM plus 10% fetal bovine serum (FBS) was used as a positive control. Data were statistically analyzed using one-way ANOVA and Newman–Keuls’s multiple comparison test, * represents a p value < 0.05** represents a p value < 0.01.
