Fig. 8
Nrf2 is associated with the eIF4E expression. (A) WB evaluated Nrf2 protein expression levels in MDAN and MDAR variants under increasing dox concentrations (0–1.6.6 µM); (B) Densitometry analysis of Nrf2. (C) WB of control eIF4E expression using siRNA on MDAN cells with respective densitometric analysis (D). (E) MMP-9 activity was evaluated by zymography after treatment with siRNA eIF4E and densitometric analysis (F). (G) WB evaluated eIF4E, p-eIF4E, and Nrf2 expression under eIF4E siRNA and concomitant treatment with dox (1.6 µM). Densitometric analysis of eIF4E (H) and p-eIF4E (I). Results showed three biological replicates mean and standard deviation (n = 3, X ± S.D.). Data were statistically analyzed using one-way ANOVA and Newman–Keuls’s multiple comparison test, *represents a p-value < 0.05, and **represents a p value < 0.01 concerning the control. GAPDH was used as a loading control for WB.
