Fig. 9
The eIF4E/ABCB1 axis in chemoresistance, the role of the 4E1RCat molecule. (A) Expression levels of ABCB1 in MDAN and MDAR cells under treatment with dox (1.6 µM) and 4E1RCat (3 µM) for 48 h through qPCR determination, GAPDH was used as a reference gene. (B) p-eIF4E expresión levels evaluated by WB under dox (1.6 µM) and 4E1RCat (3 µM) concomitant treatment, MDAN and MDAR variants were treated for 48 h. (C) Densitometry analysis of p-eIF4E; Results showed three biological replicates’ mean and standard deviation (n = 3, X ± S.D.) and expressed as % of control; data were statistically analyzed using one-way ANOVA and Newman–Keuls’s multiple comparison test ** represents a p-value < 0.01 concerning the control. GAPDH was used as a control. (D, E) Molecular docking assay of eIF4E (PDB used 4UED) under 4E1RCat and concomitant interaction of 4E1RCat and 4EBP-1. Cyan: eIF4E, Green: Tryptophan, Red: 4E Binding Protein − 1.
