Table 1 Intra- and inter-assay variability of impedance values using two differentiation protocols –Protocol I and II– in control (Ctl1) and Duchenne (DMD1) cell lines.

From: Validation of an in vitro muscle platform to evaluate myogenesis and calcium handling in control and dystrophic human myotubes

  

Protocol I

Protocol II

  

Exp 1

Exp 2

Exp 3

Intra

CV

(Mean)

Inter

CV

Exp 1

Exp 2

Exp 3

Intra

CV

(Mean)

Inter

CV

Max Conf

Ctl1 DMD1

96 ± 3.8

122.5 ± 2.6

58.2 ± 2.5

88.8 ± 3

72.6 ± 2.1

79.6 ± 2.7

3.7

3

25.2

23.3

94.5 ± 5.5

136.4 ± 6.6

112.7 ± 2.9

132.6 ± 5.2

91.6 ± 4.7

106 ± 4

4.6

4.2

11.5

13.3

1

dpd

Ctl1 DMD1

66.4 ± 3.3

72.8 ± 2.3

44 ± 2.5

52.3 ± 1.8

47.7 ± 1.4

37.2 ± 4.5

4.5

6.3

22.7

33.1

67.5 ± 3.1

82.1 ± 2.4

69.7 ± 3.1

75.5 ± 3.2

67.8 ± 2.4

71.6 ± 2.6

4.2

3.6

1.7

7

2

dpd

Ctl1 DMD1

49.6 ± 2.9

55.8 ± 2.3

45.7 ± 3.3

44.1 ± 1.6

48.4 ± 2.6

32.5 ± 3.8

6.1

6.5

4.2

26.4

65.8 ± 3.5

65.5 ± 1.7

73.8 ± 4

63.9 ± 2.5

66.4. ± 2.2

57.5 ± 2.3

4.7

3.5

6.5

6.8

3

dpd

Ctl1 DMD1

58.2 ± 5.4

68.4 ± 4.6

73.2 ± 5.3

70.2 ± 6.6

57.9 ± 10.8

48.2 ± 5.2

11.8

9

13.9

19.7

46.1 ± 3.3

53 ± 2

60.3 ± 6.2

62.9 ± 1.8

55.5 ± 4.8

59.9 ± 1.6

8.7

3.1

13.4

8.6

4

dpd

Ctl1

DMD1

35.6 ± 4.2

46.2 ± 2.9

54.2 ± 9.4

46.2 ± 3.9

49.5 ± 8.6

45.6 ± 5.3

15.4

8.8

20.9

0.7

33.6 ± 2

44.8 ± 2.2

42.1 ± 6.2

56.4 ± 2.3

51.4 ± 2.7

50.8 ± 1.7

8.7

4.1

21

11.5

  1. Values are presented as mean ± standard deviation. Intra CV: mean of the coefficient of variation (%) calculated for each experiment. Inter CV: coefficient of variation across experiment means. CV values exceeding the established thresholds, 10 and 15% for intra- and inter-assay CVs, respectively, are highlighted in Bold. Exp, experiment; Max Conf., maximal confluence; dpd, days post differentiation.
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