Fig. 5
Effect of IGF-1 signaling on amyloid burden and remodeling in Tg344-AD rats. (A–D) We characterized effects on AD-related pathology in male rats using IHC and western blotting for low molecular weight oligomers (5-10kD), but no difference was observed in amyloid burden in either hippocampus or cortex. (E,F) We further assessed amyloid plaque distribution by size in cortex and hippocampus, but there were no differences observed between control AD and Het AD males. (G–J) Similar to males, no difference was observed in amyloid burden between control and Igf1r Het AD females. (K,L) However, when plaque distribution was assessed, small plaque counts (20 to < 40uM) were increased in Igf1r het/AD female cortex (K; p < 0.05), but not in hippocampus (L). Statistics were performed either via independent samples t-test or two-way ANOVA, followed by posthoc tests with Tukey adjustment. Bar graphs represent mean ± S.E.M. *p < 0.05.
