Fig. 6

H-20 significantly upregulated PD-1 expression specifically in CGRP+ and IB4+ neuron in DRG. (A) Representative image of immunofluorescence co-staining of PD-1 with CGRP. Scale bar: 50 μm. (B) Number of the immunofluorescence cells of co-location of PD-1 with CGRP in A. n = 3. (C) Representative image of immunofluorescence co-staining of PD-1 with IB4. Scale bar: 20 μm. (D) Number of the immunofluorescence cells of co-location of PD-1 with IB4 in C. n = 3. *P < 0.05. Unpaired t tests were used for the immunofluorescence.