Fig. 2
SDS-PAGE of core proteins and functional comparison of homologous recombinase before and after sequence optimization: (A) M-marker, L1-recombinase protein, L2-single-strand binding protein, L3-promoting factor A, L4-promoting factor B, L5-DNA polymerase, L6-endonuclease lV, and L7-exonuclease lll. (B) Group a: Recombinant protein with the original homologous recombinase sequence stored at 37 °C for 0, 7, 14, and 21 days (labeled a-0, a-7, a-14, a-21). Group b: Recombinant protein with an optimized homologous recombinase sequence stored at 37 °C for 0, 7, 14, or 21 days (labeled b-0, b-7, b-14, or b-21). All samples were subsequently stored at 4 °C and subjected to comparative recombinase isothermal amplification assays.
