Table 3 Colocalization fractions (Mander’s coefficients) in the triangulenium and MT channels for dyes 1–8, averaged across 10 different images at different focal planes.

From: Microwave-assisted synthesis of new triangulenium dyes for lifetime imaging microscopy (FLIM)

Compnd.

Green channel a

Red channel b

DNA stain c

1

0.63±0.12

0.71±0.11

Partial

2

0.43±0.20

0.43±0.19

N

3

0.72±0.11

0.71±0.07

Partial

4

0.69±0.17

0.82±0.07

Partial

5

0.61±0.09

0.79±0.18

Partial

6

0.73±0.12

0.92±0.06

Partial

7

0.09±0.03

0.27±0.11

Y

8

0.15±0.08

0.25±0.09

Y

  1. a Triangulenium channel, 605/50 nm. b MT channel, 685/70 nm. c Y/N/Partial indicating whether the dye also stains nuclei and DNA.
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