Fig. 3
USP14 inhibition protects neuronal activity from OGD-induced damage in vitro. (A,B) HT-22 cells were treated with IU1 (50 μM), followed by Western blotting and semi-quantitative analysis of USP14 and apoptosis-related proteins (Bax/Bcl-2, Cleaved-caspase-3/Caspase-3, Cleaved-caspase-9/Caspase-9) post-OGD. (C,D) Stable HT-22sh-USP14 cell lines were generated, and Western blotting and semi-quantitative analysis were performed to evaluate the expression of USP14 and apoptosis-related proteins following OGD. (E) Annexin V-APC/PI double staining combined with flow cytometry was used to assess apoptosis in HT-22 cells with or without USP14 knockdown. (F) Quantification of apoptotic HT-22 cells with USP14 knockdown or control. Statistical analysis was performed using two-way ANOVA to determine p-values for the mean ± standard deviation from three independent experiments. ns p >0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
