Fig. 2

Proportion of DWV-infected adult workers over time. Initially, DWV-infected foragers constituted approximately 65%, of the observed population, which decreased significantly to less than 10% by the end of the 19-day observation period. This decline is consistent with hypotheses of DWV induced precocious foraging and subsequent virus-induced mortality in later stages of the experiment. Proportions (n = 40) were calculated from the sum of daily observed foragers (n = 361) across 4 colonies. A Binomial GLM was used, with ‘colony’ and ‘time since treatment’ as main fixed factors. The significance of the decline as a function of time since treatment was χ² = 30.90, p = 3E-8, for detailed statistical output, refer to Supplementary Table S2. The red line shows the mean model predictions, the red shaded area represents the 95% confidence interval, and the pale red dots represent the raw data points.