Fig. 5
From: Deformed wing virus affects foraging success and foraging specialization of honeybee workers
Experimental setup used to monitor foraging behaviour of deforming wing virus (DWV) infected and control bees. Bees were subjected to one of the two treatments: (1) DWV lysate + GFP control dsRNA (top left) or (2) Control virus-free lysate + DWV dsRNA (top right). Marked bees, identifiable by two different colour codes corresponding to the treatment, were introduced into the host colony via separate cages (top section of the diagram). A total of 500 bees per treatment were introduced into each of four host colonies (two colonies per replicate, replicated twice). A custom-made bottom board with a Plexiglas cover, whose key dimensions are indicated, was utilized to facilitate direct observation of marked bees entering and exiting the hive. Observations of marked foragers were made for 10 min, three times a day, over a period of 26 days.
