Table 3 Cost comparison of Sanger and minion sequencing for 96 and 960 samples of the G6PD gene.
From: Rapid detection of G6PD deficiency SNPs using a novel amplicon-based minion sequencing assay
Category | Description | Cost per sample (USD) | Cost for 96 samples (USD) | Cost for 960 samples (USD) |
|---|---|---|---|---|
Sanger Sequencing | PCR purification + sequencing (7 fragments × Fw & Rv) | $7.90 | $8,131 (no discount) | $81,312 (no discount) |
Estimated discount (10–15%) | $72.00–$76.23 | $6,915 – $7,318 | 69,120–$73,180 | |
MinION Sequencing | Consumables (flowcells, kits, barcoding, ligation, wash kits) | $12.29 | $1,180 | $11,805 |
AMPure XP beads purification (additional cost) | $11.03 | $1,058 | $10,588 | |
One-time MinION Mk1C Starter Pack | $4,900 | $4,900 | ||
Total MinION cost | First run (consumables + beads + sequencer) | $7,138 | $27,293 | |
Subsequent runs (consumables only, excluding beads) | $1,180 | $11,805 | ||
Subsequent runs (including beads) | $2,238 | $22,393 |
