Table 3 Primer information for examining PPARγ gene expression.
Target gene | Product size (bp) | Tm (°C) | Primer sequence (5′→3′) | Direction | Source/References |
|---|---|---|---|---|---|
PPARγ | 150 | 60 | AGGCCGAGAAGGAGAAGCTG | Forward | Designed using NCBI Primer-BLAST |
TGGCCACCTCTTTGCTCTA | Reverse | Designed using NCBI Primer-BLAST | |||
β-actin | 120 | 60 | AGAGGGAAATCGTGCGTGAC | Forward | Designed using NCBI Primer-BLAST |
CAATAGTGATGACCTGGCCGT | Reverse | Designed using NCBI Primer-BLAST | |||
GAPDH | 123 | 62.6 | AGGTCGGTGTGAACGGATTTG | Forward | PrimerBank ID: 6679937a1 |
GAPDH | 123 | 60.2 | TGTAGACCATGTAGTTGAGGTCA | Reverse | PrimerBank ID: 6679937a1 |
