Fig. 1
Survival of hypoxia and antimycin A challenge in the hydrothermal vent xtcrabs. (a) Aquarium xtCrabs (Xenograpsus testudinatus) and control tdcrabs (Thranita danae) collected from their original habitats (hydrothermal vent and intertidal zone, respectively) were maintained for one month in the normal seawater aquarium for acclimation. Then aquarium xtcrabs and control tdcrabs were subjected to a severe hypoxic challenge (Dissolved oxygen, DO = 0.19 mg/L seawater continuously flushed with nitrogen gas. DO of the normoxia group = 8.31 mg/L). The activity levels of xtcrabs and tdcrabs, including movement, touching reaction, and body calibration were checked every 4 h over 24 h period (n = 9 crabs/group). (b) LC50/96 hr of antimycin A was tested in aquarium xtcrabs. The aquarium xtcrabs were exposed to various antimycin A concentrations (0 ppb, 1 ppb, 10 ppb, 100 ppb, 500 ppb, 1000 ppb) for 96 h. Survival was recorded every 24 h for each group (n = 9 xtcrabs/group). (c) Oxygen consumption rate (mg O2 g− 1 hr− 1) in xtcrab under different antimycin A treatments for 30 min were examined. Means ± SD (n = 3 xtcrabs/group). Asterisks indicate the significant difference between 0 ppb and 400 ppb group by t-test (*: p < 0.05). (d) Oxygen content in a sealed water chamber for each xtcrab was recorded under 0 ppb antimycin A treatment for 30 min. (e) Oxygen content in a sealed water chamber for each xtcrab was recorded under 400 ppb antimycin A treatment for 30 min.
