Table 1 Statistical optimization of the HA-REG-CAP-CUBs formulation via design Expert®.
Run | Factor 1 | Factor 2 | Factor 3 | Response 1 | Response 2 | Response 3 | Response 4 | Response 5 |
|---|---|---|---|---|---|---|---|---|
GMO (mg) | Tween 80 (mg) | CAP (mg) | Particle size (nm) | PDI | Zeta potential (mV) | %EE (REG) | %EE (CAP) | |
01 | 200 | 30 | 5 | 244.1 ± 2.4 | 0.239 ± 0.03 | −34.2 ± 3.1 | 82.47 ± 1.3 | 75.39 ± 2.1 |
02 | 150 | 40 | 5 | 187.4 ± 3.1 | 0.147 ± 0.01 | −16.3 ± 2.5 | 72.48 ± 1.2 | 64.38 ± 1.6 |
03 | 200 | 40 | 10 | 234.7 ± 4.3 | 0.256 ± 0.01 | −28.2 ± 4.5 | 80.66 ± 1.4 | 83.57 ± 1.2 |
04 | 100 | 40 | 10 | 156.4 ± 5.2 | 0.167 ± 0.02 | −10.9 ± 2.3 | 63.01 ± 0.9 | 65.44 ± 2.2 |
05 | 200 | 20 | 10 | 252.3 ± 2.4 | 0.227 ± 0.03 | −38.1 ± 3.4 | 81.47 ± 1.1 | 82.35 ± 1.5 |
06 | 150 | 30 | 10 | 199.4 ± 2.5 | 0.269 ± 0.01 | −24.2 ± 3.7 | 74.59 ± 1.5 | 73.66 ± 1.7 |
07 | 100 | 30 | 15 | 183.6 ± 3.7 | 0.186 ± 0.02 | −12.7 ± 3.1 | 59.36 ± 1.3 | 69.38 ± 1.3 |
08 | 150 | 40 | 15 | 213.1 ± 2.6 | 0.177 ± 0.02 | −20.4 ± 4.8 | 71.49 ± 1.6 | 76.28 ± 2.3 |
09 | 150 | 30 | 10 | 196.1 ± 1.4 | 0.231 ± 0.03 | −25.5 ± 5.2 | 78.56 ± 2.1 | 76.48 ± 2.5 |
10 | 150 | 20 | 15 | 215.3 ± 3.2 | 0.326 ± 0.02 | −19.7 ± 3.3 | 72.33 ± 2.3 | 78.69 ± 1.9 |
11 | 200 | 30 | 15 | 258.1 ± 4.2 | 0.247 ± 0.01 | −32.8 ± 2.3 | 80.46 ± 0.6 | 84.69 ± 0.8 |
12 | 150 | 30 | 10 | 198.6 ± 5.5 | 0.251 ± 0.02 | −26.1 ± 1.8 | 76.39 ± 1.3 | 78.81 ± 1.4 |
13 | 100 | 20 | 10 | 176.7 ± 4.6 | 0.269 ± 0.04 | −18.3 ± 2.5 | 64.68 ± 1.6 | 67.58 ± 2.3 |
14 | 100 | 30 | 5 | 173.6 ± 2.8 | 0.167 ± 0.05 | −9.78 ± 1.3 | 61.89 ± 1.1 | 59.08 ± 1.5 |
15 | 150 | 20 | 5 | 210.4 ± 3.9 | 0.273 ± 0.04 | −18.9 ± 1.7 | 73.97 ± 1.9 | 71.49 ± 1.7 |
