Fig. 6
Effect of Sal B on miR-23a/PTEN signaling pathway in vivo. (A) RT-PCR analysis of miR-23a in the tumor tissues of mice treated with Sal B. (B) Western blot analysis of PTEN and p-AKT in the tumor tissues of mice treated with Sal B. The grouping of gels/blots were cropped from different parts of the same gels. (C) The protein levels PTEN was quantified by ImageJ and normalized to the β-tubulin protein content. (D) The protein levels p-AKT was quantified by ImageJ and normalized to the β-tubulin protein content. The bar graphs present the mean ± SD. Original blots/gels are presented in Supplementary Figure S5. Statistical analysis was performed by one-way ANOVA. **P < 0.01 and *P < 0.05 vs. control group.
