Fig. 8
From: SLC45A2 drives prostate cancer progression through tumor promotion and immune suppression
Analysis of signaling pathways and immune cell co-culture assays. (A) Western blot analysis of p-PI3K, PI3K, p-AKT, AKT, AMACR, PCNA, and GAPDH protein levels in DU145 cells. (B) Western blot analysis of p-PI3K, PI3K, p-AKT, AKT, AMACR, PCNA, and GAPDH in control and SLC45A2-knockdown DU145 cells treated with IGF-1. (C) Transwell migration assay assessing the migratory ability of control and SLC45A2-knockdown DU145 cells following IGF-1 treatment (**P < 0.01). (D) CCK-8 assay evaluating the proliferative capacity of control and SLC45A2-knockdown DU145 cells treated with IGF-1. (E) Effects of SLC45A2 knockdown combined with CD8⁺ T-cell co-culture on DU145 cell proliferation. Viable cells were visualized by crystal violet staining; purple-stained cells represent surviving DU145 cells. Differences in staining intensity and distribution reflect cell viability under various treatment conditions. “–” indicates absence of treatment; “+” indicates presence of treatment.
