Fig. 1

UV-visible absorption and fluorescence spectroscopic characterization of Erythrosin B-ganciclovir interaction. (A) UV-visible absorption spectra of Erythrosin B, ganciclovir, and their complex showing hypochromic and hypsochromic effects upon complex formation. (B) Fluorescence emission spectrum of Erythrosin B at λex = 530 nm showing maximum emission at 555 nm. (C) Fluorescence quenching of Erythrosin B upon successive addition of ganciclovir concentrations (0–3.0 µg/mL) at λex = 530 nm and λem = 555 nm.