Table 1 The primers used for the amplification of target fragments of genes in the present study.

From: Molecular survey and phylogenetic analysis of Borrelia theileri, Rickettsia aeschlimannii, Mycoplasma ovis, and Mycoplasma wenyonii in sheep and goats from southern Egypt

Pathogen

Target gene

Sequence (5’−3’)

Product size (Pb)

References

Borrelia theileri

Flagellin (flaB) gene

GATCA(G/A)GC(T/A)CAA(C/T)ATAACCA(A/T)ATGCA

AGATTCAAGTCTGTTTTGGAAAGC

344

(Takano et al., 2010)

GCTGAAGAGCTTGGAATGCAACC

TGATCAGTTATCATTCTAATAGCA

Rickettsia aeschlimannii

Citrate synthase (gltA) gene

ATGACCAATGAAAATAATAAT

CTTATACTCTCTATGTACA

341

(Mediannikov et al., 2004)

GGGGGCCTGCTCACGGCGG

ATTGCAAAAAGTACAGTGAAC

Mycoplasma species

16S rRNA

ATACGGCCCATATTCCTACG

TGCTCCACCACT TGT TCA

595–618

(Criado-Fornelio et al., 2003)

Back to article page