Table 1 Identified substances and metabolites in snake venom after subcutaneous injection of ketamine and pilocarpine to Heterodon nasicus nasicus for venom milking.
Substance | Ion M + H+ sum formula | m/z M + H+ theo. mass | m/z M + H+ obs. mass | Mass error in ppm | mSigma | Tandem-MS confirmation | Isotope ratio 37Cl:35Cl |
|---|---|---|---|---|---|---|---|
Ketamine | C13H17ClNO | 238.0993 | 238.0994 | -0.3 | 7.5 | ✓ | 32% |
Phenol-ketamine | C13H17ClNO2 | 254.0942 | 254.0945 | -1.0 | 77.6 | ✓ | 32% |
Hydroxy-ketamine | C13H17ClNO2 | 254.0942 | 254.0942 | 0.3 | 40.6 | ✓ | 26% |
Norketamine | C12H15ClNO | 224.0837 | 224.0838 | -0.6 | 42.1 | ✓ | 26% |
Phenol-norketamine | C12H15ClNO2 | 240.0786 | 240.0789 | -1.2 | 30.7 | ✓ | 29% |
Hydroxynorketamine | C12H15ClNO2 | 240.0786 | 240.0789 | -1.3 | 20.2 | ✓ | 30% |
Dehydronorketamine | C12H13ClNO | 222.0680 | 222.0680 | 0.2 | 51.6 | ✓ | 26% |
Phenol-hydroxy-/ dihydroxy-norketamine | C12H15ClNO3 | 256.0741 | 256.0734 | 0.6 | n.a. | - | 37% |
Pilocarpine | C11H17N2O2 | 209.1285 | 209.1286 | -0.6 | 3.9 | ✓ | n.a. |
Pilocarpic acid | C11H19N2O3 | 227.1390 | 227.1393 | -1.1 | 2.4 | ✓ | n.a. |
