Fig. 2
From: Metabolic profiling of therapy-induced senescent cancer cells via TPEF, MALDI-MS, and RNA-sequencing
The metabolic alterations occurring in cells following the onset of the senescent state can be visualized in a completely label-free fashion. (A) Multimodal TPEF (green) images of control and senescent [radiation and doxorubicin induced) accompanied by the classical transmission image. Scale bar: 20 μm. (B) The percentage of cell area occupied by NAD(P)H and FAD coenzymes in the different cancer cell type measurements, compared by condition of the cells: control and senescent (radiation- and doxorubicin-induced). Two-sided Mann-Whitney U tests were performed for statistical significance: *0,01 < p ≤ 0,05; **p ≤ 0,01; ***p ≤ 0,001; ****p ≤ 0,0001. (C) The Aggregation Index results for NAD(P)H and FAD coenzymes in the different cancer cell lines measurements, compared by condition of the cells: control and senescent (radiation- and doxorubicin-induced). Two-sided Mann-Whitney U tests were performed for statistical significance: *0,01 < p ≤ 0,05; **p ≤ 0,01; ***p ≤ 0,001; ****p ≤ 0,0001. This Figure was included in Dr. Silvia Ghislanzoni’s PhD thesis Investigation of Therapy-Induced Senescent Cancer Cell Phenotypes with Mass Spectrometry and Non-Linear Optical Microscopy (https://doi.org/10.21954/ou.ro.00101782)
