Table 1 Summary of purification of enzymes at different steps.
From: Multi enzyme production and alginate encapsulation from Bacillus subtilis for stable feed additives
Enzymes | Purification steps | Activity (U/mL) | Protein content (mg/mL) | Specific activity (IU/mg of protein) | Purification (fold) |
|---|---|---|---|---|---|
Protease | Crude enzyme | 6.25 | 4.84 | 1.29 | 1 |
(NH4)2SO4 precipitation | 4.375 | 0.32 | 13.7 | 6.34 | |
Dialysis | 3.187 | 0.17 | 18.3 | 12.67 | |
Sepharose CL-6B | 2.125 | 0.08 | 26.4 | 87.5 | |
Lipase | Crude enzyme | 20.45 | 1.218 | 16.78 | 1 |
(NH4)2SO4 precipitation | 14.29 | 0.45 | 31.62 | 5.9 | |
Dialysis | 12.09 | 0.309 | 39.08 | 7.63 | |
Sepharose CL-6B | 6.98 | 0.084 | 82.73 | 42.6 | |
Cellulase | Crude enzyme | 85.76 | 12.06 | 7.11 | 1 |
(NH4)2SO4 precipitation | 27.98 | 1.905 | 14.68 | 2.06 | |
Dialysis | 5.87 | 0.175 | 33.5 | 31.22 | |
Sepharose CL-6B | 4.65 | 0.054 | 85.3 | 68.12 | |
Amylase | Crude enzyme | 9.02 | 0.673 | 13.4 | 1 |
(NH4)2SO4 precipitation | 6.05 | 0.212 | 28.5 | 18.7 | |
Dialysis | 5.67 | 0.151 | 37.4 | 21.09 | |
Sepharose CL-6B | 1.63 | 0.029 | 56.2 | 89.03 |
