Fig. 5
Physical interaction between eIF3f and the autophagy vesicle marker LAMP1. (A) Protein–protein functional enrichment of the “core eIF3f-eIF3” used for normalization and identification of positively enriched interactors in MT (B) Western blot detection of eIF3f and LAMP1 after immunoprecipitation from parental MT (day 6) lysates. Anti-eIF3f or anti-LAMP1 antibodies were used for precipitation as indicated and corresponding isotype (Rabbit IgG isotype) was used as control. eIF3a protein detection was used as a positive control for anti-eIF3f immunoprecipitation and 4% input whole cell lysate (WCL) was used as an internal control for protein detection. Original blots are presented in Supplementary Fig. 14. (C) Immuno-detection of eIF3f and LAMP1 visualised with anti-rabbit 488 and anti-mouse 568 coupled secondary antibodies respectively in parental differentiated (day 6) myotubes (MT). DNA was stained with Hoechst. Dashed rectangles are displayed in a magnified rectangle to appreciate co-localisation of both proteins in the cytoplasmic part of MT.
