Table 2 Primers used in the study.
CRISPR primers | Sequences (5′–3′) | mPAM |
|---|---|---|
Pam-sg1-F | TAGGTGCTATCTGTAACTCG | AGG to AGT |
Pam-sg1-R | CGAGTTACAGATAGCACCTA | Â |
Pam-sg2-F | CTAGTCTATAAGTGTATCAG | GGG to GTT |
Pam-sg2-R | CTGATACACTTATAGACTAG | Â |
EIF3F_scrn_F | TTGAGACCATGCTCAACAGCAAC | Â |
EIF3F_scrn_R | GTGTACTGCTTGGGGTCCA | Â |
Primer CpEIF3FGWF | GGGGACAAGTTTGTACAAAAAAGCAGGCTACCATGGCCACACCGGCGGTACCAGTAAGT | Â |
Primer CpEIF3FGWR | GGGGCACTTTGTACAAGAAAGCTGGGTACAGGTTTACAAGTTTTTCATTGAGTGC | Â |
Primer NpEIF3FGWF | GGGGACAAGTTTGTACAAAAAAGCAGGCTCCACACCGGCGGTACCAGTAAGTGCT | Â |
Primer NpEIF3F GWR | GGGGACCACTTTGTACAAGAAAGCTGGGTCTACAGGTTTACAAGTTTTTCATTGAG | Â |
AgeI-EIF3F FWD | CCA/CCGGTATGGCCACACCGGCGGTACCAGTAAG | Â |
EIF3F-BamHI | GCGGGATC/CCAGGTTTACAAGTTTTTCATTGAGTGCA | Â |
XhoI-EIF3F FWD | CGCC/TCGAGGCCACACCGGCGGTACCAGTAAGTG | Â |
EIF3F-HindIII | GGGAAGCT/TTCACAGGTTTACAAGTTTTTCATTGAGTGCA | Â |
