Fig. 15

Photomicrograph T.S. of Eleutherocaulis alte showing the suprapedal gland stained with: (A) and (B) PAS showing the highly positive reaction of type II only. (C-E) and (F-H) Combined PAS & Alcian blue and toluidine blue, respectively, showing that type I secretes neutral mucopolysaccharides (orthochromatic) and type II are mixed glands that secrete acid mucopolysaccharides (metachromatic). (I-K) The bromophenol blue showed that the protein secretion was restricted to type I of the gland.