Table 2 Experimental design for comparative analysis of ureolytic bacterial growth characteristics.

From: Strengthening mechanisms of indigenous bacteria in granite residual soil improvement via microbial induced calcite precipitation

Influencing parameters

Other culture conditions

Culture medium

(YE, LB, 907, AN)

Temperature: 30℃, Inoculation volume: 2%, Culture medium pH value: 8.0, Shaker frequency: 200 rpm.

Inoculation volume (%)

(1, 2, 3, 4)

Culture medium: LB, Temperature: 30℃, Culture medium pH value: 8.0, Shaker frequency: 200 rpm.

Culture medium pH value

(6.0, 7.0, 8.0, 9.0, 10.0)

Culture medium: LB, Temperature: 30℃, Inoculation volume: 2%, Shaker frequency: 200 rpm.

Temperature (℃)

(20, 25, 30, 35, 40)

Culture medium: LB, Inoculation volume: 2%, Culture medium pH value: 8.0, Shaker frequency: 200 rpm.

Shaker frequency (rpm)

(150, 200, 250)

Culture medium: LB, Temperature: 30℃, Inoculation volume: 2%, Culture medium pH value: 8.0.

  1. Note: Culture medium YE is comprised yeast extract (20 g/L), ammonium chloride (10 g/L), and nickel chloride (0.05 g/L); culture medium LB comprised peptone (10 g/L), yeast extract (5 g/L), and sodium chloride (10 g/L); culture medium 907 comprised peptone (10 g/L), beef extract (10 g/L), sodium chloride (5 g/L), urea (20 g/L); culture medium AN comprised peptone (10 g/L), beef extract (10 g/L), sodium citrate (anhydrous) (5 g/L), urea (20 g/L), nickel chloride (0.05 g/L).
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