Fig. 2 | Scientific Reports

Fig. 2

From: Novel fully human IgG1 targeting folate receptor α demonstrates antitumor efficacy driven by avidity rather than monovalent binding affinity

Fig. 2Fig. 2

Flow cytometry analysis of AFRA hIgG1 and ChiMOv19 binding ability and specificity. (a) Histograms of mean fluorescence intensities (MFI) of ovarian cancer cell lines and primary cultures of ovary cancer as reported in Supplementary Table S2a. (b) Histograms of MFI across multiple cell lines, as reported in Supplementary Table S2b. Two different sizes for y axis scales (bottom 0 < MFI < 2000 and top 3000 < MFI < 50000) were used to better appreciate the cell lines that have a low MFI. The red bar corresponds to ChiMOv19; blue bar corresponds to AFRA hIgG1. * p < 0.05; ** p < 0.01; *** p < 0.001; ns: not statistically significant. (c) Representative flow cytometry histograms showing AFRA hIgG1 compared to ChiMOv19 binding on highly FRα expressing cell lines (IGROV1 and A431tFR, top left and right, respectively) and FRα negative cells (A431 and A431 tMock, bottom left and right respectively) cell lines. Light blue histograms correspond to AFRA hIgG1 and ChiMOv19 binding; red histograms correspond to a negative control antibody (Alexa 488 anti-human IgG (H + L) antibody).

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