Fig. 3
24 h-old biofilm-derived parameters obtained under the experimental conditions indicated in Material and Methods for P. aeruginosa PAO1 grown on plastic plates. (A) Absorbance of samples after CV assays: columns represent the mean values of eight wells from each of four independent plate replicates, with error bars corresponding to SDs. (B) biomass data were obtained by analyzing cyan fluorescence (PAO1-Cfp, filled columns) while eDNA values by staining with Di-YO1 (excitation 491 nm-emission 508 nm, stripe pattern). Columns represent the mean values from at least three independent experimental replicates while the error bars correspond to SDs. (C) Quantification of PAO1 cells viability in 24 h-old biofilms untreated, treated with BZs or solvent, measured through the BacTiterGlo assay. The negative control column corresponds to the media without bacteria. Columns represent the mean values of the experimental replicates, with error bars corresponding to SDs. RLU: relative luminiscence units. (D) Production of c-di-GMP by PAO1 in response to BZs: light output was quantified as a function of growth (RLU/OD600) for PAO1 PcdrA::luxCDABE. Representation of results was performed by calculating the area under the curve (AUC) of RLU/OD600 values for the 24 h period, and displayed through columns and error bars (SDs), from three independent experimental replicates. Statistical significance was determined by one-way ANOVA plus Tukey’s post-hoc test.*P < 0.05. n.s.: not significant.
