Fig. 1
Profile of established mAbs. (a) Microscopic images of NK lymphoma cells after incubation with antibodies. Representative microscopic images of NK lymphoma cell lines treated with hybridoma culture supernatants. Control: culture medium alone. (b) Time- and dose-dependent cytotoxicity of mAb ANAP against NK/T lymphoma cell lines. Cells were treated with 3 µg/mL mAb ANAP for indicated times (left) or with indicated concentrations for 120 min (right). Viability was determined by dye exclusion test (mean ± SD, n = 3). (c) Dose-response curve of mAb ANAP against NKL cells. NKL cells were treated with various concentrations of monoclonal antibody ANAP, and cell viability was assessed. The percentage of alive cells was plotted against the logarithmic concentration of mAb ANAP (µg/mL). The half-maximal effective concentration (EC50) was determined to be 0.12 µg/mL, indicating the concentration required to achieve 50% cell death. Data points represent mean values.
