Table 8 Assessment of the greenness of the developed voltammetric method and HPLC method using AGREE calculator.
Parameters | Developed voltametric method | Reported HPLC3 |
|---|---|---|
1- Select the sampling procedure | off-line | off-line |
Enter the amount of sample in gm. or Ml | 0.015 g | 0.05g |
What’s the positioning of the analytical device | Off-line | Off-line |
How many major distinct steps are there in the sample preparation procedure? (sonication, mineralization, centrifugation, derivatization, etc.) | 3 or fewer | 3 or fewer |
Degree of automation, sample preparation | Semi-automatic miniaturized | Semi-automatic, Not miniaturized |
Select derivatization agent (if used) | Not used | Not used |
Enter the amount of waste in g or mL | < 0.1g | < 0.1g |
Number of analytes determined in a single run-sample throughput (sample analyzed per hour) | 1 (analytes) 60 | (analytes) 4 |
Select the most energy-intensive technique used in the method Total power consumption of a single analysis in kWh | < 0.001 kWh | 0.1–1.5 kWh |
Select the type of reagent | No reagent | All reagents are bio-based |
Does the method involve the use of toxic reagents of solvents | No | Yes Acetonitrile–trifluoracetic acid |
Select the threats which are not avoided | No threats | Highly flammable |
