Table 9 Calculation of the analytical Eco-Scale score for the developed RP-HPLC method for IMG assay.
Parameters | Penalty Points (PPs) | |||
|---|---|---|---|---|
Reagents | ||||
Type | Hazard | Amount | Subtotal PPs | |
Ethanol | two pictograms, danger | 10–100 mL | 4*2 | 8 |
Octane sulfonic acid | one pictogram, danger | 1 mL | 2*1 | 2 |
Total PPs for reagents (∑ hazard* amount) | 10 | |||
Instrument | ||||
Energy consumption | ≤ 1.5 kW/h (1) | 1 | ||
Emission of vapors or gasses | none | 0 | ||
Waste | ||||
Waste generated | > 10 mL | 5 | ||
Waste treatment | No treatment | 3 | ||
Total PPs for waste | 8 | |||
Total PPs | 19 | |||
Analytical Eco-Scale Score (= 100-PPs) | 81 | |||
