Fig. 1

Glioma stem cell (GSC) proliferation and therapeutic response profiles. (A) GSC culture experimental workflow and culture color code used throughout. (B) Dark field microscopy of GSC cultures in mid-exponential growth in serum-free medium and 5% oxygen on untreated plastic. Scale bars are 200 μm long. (C) Serial cell counting reveals different GSC culture population doubling rates. (D) Colony-forming efficiencies (CFE) of untreated GSC cultures determined by dilution cloning. (E) GSC culture CFE as a function of IR or TMZ dose versus untreated controls. Abbreviations: CellTag, lentiviral molecular barcoding system; IR, ionizing radiation; mtDNA, mitochondrial DNA; TMZ, temozolomide; scRNAseq, single cell RNA sequencing; WES, whole exome sequencing. Error bars represent standard error of the mean in replicate experiments.