Table 1 The selected optimum parameters for the reaction between BER and fluorescamine.

From: Utility of fluorescamine for spectrofluorimetric quantitation of berotralstat hydrochloride in spiked human plasma and content uniformity test and evaluation of method greenness

Parameter

Optimum value

pH

7.5

Buffer volume

1.0 mL

Fluorescamine volume

1.2 mL

Diluting solvent

Ethanol

Time

10 min

Molar ratio

1:1

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