Table 5 Robustness study of the proposed spectrofluorometric method for determination of BER.

From: Utility of fluorescamine for spectrofluorimetric quantitation of berotralstat hydrochloride in spiked human plasma and content uniformity test and evaluation of method greenness

Optimization factor

Value

% Recoverya ± RSD

Borate buffer pH

7.4

99.53 ± 1.11

7.5

100.53 ± 0.99

7.6

98.49 ± 1.21

Borate buffer volume (mL)

0.9

99.43 ± 1.53

1.0

99.27 ± 0.67

1.1

99.30 ± 1.46

Fluorescamine volume (mL)

1.3

100.47 ± 1.85

1.4

100.90 ± 1.25

1.5

100.00 ± 1.14

  1. a The values are the average of three determinations.
Back to article page