Fig. 7 | Scientific Reports

Fig. 7

From: The contribution of the membrane-bound complement regulatory proteins CD46 and CD55 in phases of acute lymphocytic leukemia and acute myelogenous leukemia

Fig. 7

ShRNA-mediated combined knockdown of CD46 and CD55 mCRPs expression on HSB-2 cells compared to the mock transfected controls. (A) Co-silencing of CD46 and CD55 expression in transfected cells led to a highly significant reduction in the expression of both proteins(p < 0.001). A significant difference was observed between CD46 and CD55 knockdown(p < 0.05).The percentage of inhibition was calculated relative to nonsilencing shRNA controls (= 100%). p < 0.001(***), p < 0.05(*). (B) Flow cytometry analysis chart for CD46 and CD55 expression on HSB-2 cells transfected with combined CD46 and CD55 shRNA silencing plasmids. Dots in the third quadrant show the double negative expression for both CD46 and CD55 after knockdown of both CD46 and CD55 genes using combined CD46 and CD55 silencing shRNA plasmids.

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