Fig. 8 | Scientific Reports

Fig. 8

From: The contribution of the membrane-bound complement regulatory proteins CD46 and CD55 in phases of acute lymphocytic leukemia and acute myelogenous leukemia

Fig. 8

Acute lymphocytic leukemia cells viability after silencing of mCRP. Viability of HSB-2 cells was analyzed after silencing of CD46 and CD55 protein expression separately as well as combined silencing of both proteins using shRNA plasmids. Cell viability was assessed using MTT reagent in the presence of NHS as a source of complement to mimic exposure to physiological complement. Cells had a reduced viability in the absence of CD46 (p < 0.001) and CD55 (p < 0.01) as well as in CD46 and CD55 co-silenced cells (p < 0.01). These results demonstrate that CD46 and CD55 contribute to the protection of leukemic cells against complement attack, and that their complete depletion sensitizes cells to CDC, which directly links this assay to their contribution in immune escape. P < 0.01 (**), p < 0.001 (***).

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