Table 1 Oligonucleotide sequences.
Namea | Sequence (5′ to 3′)b | Usage |
|---|---|---|
miR-30a-5p mimics | UGUAAACAUCCUCGACUGGAAG | Transfection |
Inhibitor (sense) | CUUCCAGUCGAGGAUGUUUACA | Transfection |
si429 (sense) | CUGGCUUCUUGUCCUUUAUTT | Transfection |
si1485 (sense) | CAGCCAUAUACCAGGAAUATT | Transfection |
U6-F | CTCGCTTCGGCAGCACA | qRT-PCR |
U6-R | AACGCTTCACGAATTTGCGT | qRT-PCR |
PHTF2-F | ATGGCGTCCAAAGTCACAGA | qRT-PCR |
PHTF2-R | CTACATTATACCCACCTGTT | qRT-PCR |
miR-30a-5p-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCTTCCA | RT |
miR-30a-5p-Q | CGCGTGTAAACATCCTCGAC | qRT-PCR |
GAPDH-F | AAATCCCATCACCATCTTCC | qRT-PCR |
GAPDH-R | TCACACCCATGACGAACA | qRT-PCR |
PHTF2-wt-F | tgtttaaacgagctcgctagcCAATTCAAAGAAAAGAAGATGTAGCC | plasmid construction |
PHTF2-wt-R | cttgcatgcctgcaggtcgacCACAATTTGTTATTTTGCCAATTATTT | plasmid construction |
PHTF2-mut-F | TGAAGcagcgctATCAGACTGTCTTGTGCAATTCTTATATT | mutagenesis |
PHTF2-mut-R | CTGATagcgctgCTTCAACTCCCTAGCTGAGACTCC | mutagenesis |
PHTF2-F | cttggtaccgagctcggatccATGGCGTCCAAAGTCACAGATG | plasmid construction |
PHTF2-R | aacgggccctctagactcgagTCATGACTTAATCTTCCATAGCTTTAAA | plasmid construction |
