Fig. 3
From: Tissue resident memory T cells populate the human uveal tract
TRM cells populate human aqueous humour and uveal tissue during active uveitis. (A) Experimental overview for scRNA sequencing and T cell receptor profiling of cells in aqueous humour during uveitis. (B) Uveitis diagnosis and HLA-B27 status of aqueous humour donors. UMAP plots of (C) all cells with broad cell types annotated and (D) T cell subtypes following subsclustering of T cells only. (E) Analysis to different diversity metrics to assess diversity of T cell receptor repertoire across T cell subtypes. (F) Proportions and (G) repertoire space of expanded T cell receptor clonotypes. (H) For each donor, cells expressing the most abundant clonotype are highlighted. Corresponding TRA and TRB chains of abundant clones are specified. (I) Comparison of T cell subtype proportions between all T cells, single clones and expanded (present in ≥ 2 cells) clones. (J) Comparison of egress, migration, and tissue retention markers between expanded and non-expanded T cells. (K) Immunofluorescence imaging of CD3, CD8 and CD103 cells in enucleated eyes from donors with advanced uveitis donors alongside the vascular marker CD31.
