Fig. 4
Cells 10 Days PTR decrease both mitochondrial fission and fusion dynamics. (a) Representative fluorescence images of mitochondria every 60 s for 6 min in untreated cells and cells 10 Days PTR. Yellow arrow and box indicate a fusion event, while a blue arrow and box indicate a fission event. (b) Linear regression analysis of fission rate vs mitochondrial area. (c) Linear regression analysis of fusion rate vs mitochondrial area. (d) Quantification of fission rate in untreated cells and cells 10 Days PTR when normalized to mitochondrial area. (e) Quantification of fusion rate in untreated cells and cells 10 Days PTR when normalized to mitochondrial area. n = 37 and 17 cells for untreated and cells 10 Days PTR, respectively (b–e). Data are presented as scatter plots (b, c) and mean ± s.e.m. (d, e). R-squared values were calculated with the lm() and summary() functions in RStudio93 (b, c). p values were calculated with the lm() and summary() functions in RStudio93 (b, c) and a two-tailed Mann–Whitney test (d, e). * p < 0.05, ** p < 0.01.
