Fig. 5
Oma1 knockdown promotes cell death in cancer cells surviving chemotherapy. (a) Representative western blot of OMA1 after 72 h of siRNA or Scramble transfection in untreated cells and cells 10 Days PTR. (b) Quantification of OMA1 protein expression in untreated cells after 72 h of siRNA or Scramble transfection. (c) Quantification of OMA1 protein expression in cells 10 Days PTR after 72 h of siRNA or Scramble transfection. (d) Representative images of one well of a 96-well plate of Hoechst-stained nuclei from untreated cells and cells 10 Days PTR after 72 h of siRNA or Scramble transfection. (e) Quantification of cell viability normalized to scramble negative control in untreated cells. (f) Quantification of cell viability normalized to scramble negative control in cells 10 Days PTR. n = 3 biological replicates (b, e, f); n = 4 biological replicates (c). Data are presented as mean ± s.e.m. (b, c, e, f). p values were calculated with a two-tailed unpaired t-test (b, c, e, f). ns not significant, * p < 0.05, ** p < 0.01, *** p < 0.001.
